Animals were administered a single i.p. dose of either mAb (100 to 150 µg/0.5 ml, as specified in single experiments) and, 2 h later, received a systemic challenge with C. albicans (5×105 or 106 cells/0.2 ml, i.v .. Control animals were treated with the same doses of an irrelevant, anti-CRM IgG2b mAb.
The above mouse strains were maintained on a C57BL/6 background, and both male and female mice in the age range of postnatal days 18 50 were used as specified in individual experiments.
Cells were treated with 200 µM H2O2 for 1 h, then washed twice with serum-free medium to remove H2O2, and either re-cultured in fresh complete medium for various durations as specified in individual experiments or, when indicated, treated with 5 µM SB203580 (Sigma, USA) for 40 min at 37°C.
Confluent cells were then preconditioned for 24 hours with the same medium containing 0.5% FBS and then incubated in the presence of 50 nM 1,25-dihydroxyvitamin D3 (VitD3; Sigma-Aldrich, St-Quentin Fallavier, France) or of the vehicle (ethanol 96%, 1 μl/ml) under normoxia (20% O2) or hypoxia (2% O2) for different periods of time as specified in the experiments.
Timelapse start and duration was as specified in the experiment.
CD34+ cells were thawed, counted, assessed for viability and seeded at 0.5 to 2×105 cells/ml in various culture media (as specified in each experiment).
RNA was also extracted from different organs of plant seedlings as specified in each experiment.
Media for adults used this standard diet except with yeast at concentrations at 1%, 2%, 4 %, 8 %, 12or 16% w/v as specified in each experiment.
Zinc depletion and abundance were obtained by supplementing media with ethylenediamine tetraacetic acid (EDTA) or ZnCl2, respectively, as specified in each experiment.
Each of the 20 cell lines was exposed for 96 h to concentrations of PD0325901 and AKT 1/2 kinase inhibitor to inhibit signalling of p-S6 and p-ERK by different degrees, as specified in each experiment.
Various PCa cell lines, including LNCaP C-33/C-81 C-33/C-81 C-33/C-81nd MDA PCa2b AS/AI ceLNCaPere C4-2/C4-2B C4-2/C4-2B C4-2/C4-2B × 10 cells/well, respectively, in 6-well plands in regular MDAium for 3 days and then treated with 1 mM VPCa2b different concentrAS/Ans of HDAC Inhibitors as specellsd in each experiment for 2 days.
