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To perform these, the differential equation is repeatedly applied at regular discrete time-steps, with parameters as specified in figure captions in each case.
Specifically, we affix labels to atoms and bonds as specified in Figure 2. It should be noted that triple bonds would also be labelled as "=".
After treating the cells as specified in figure legends, the Caspase-Glo 3/7 reagent was added to the wells followed by measurements of luminescence 1 h later with a plate-reading luminometer (GloMax® 96 Luminometer, Promega).
We pre-amplified samples by treating 50 GFP-positive cells per mESC line per time point following manufacturer's protocol (Fluidigm Corporation) using 20× Taqman gene expression assays (Applied Biosystems Tablee S2) as specified in Figure 8 and Table S2.
IκBα-forward: 5'-GACCTGGTGTCACTCCTGTTG; IκBα-reverse: 5'-CTCTCCTCATCCTCACTCTCTGG; GAPDH-forward: 5'-GAAGGTGAAGGTCG GAGTC; GAPDH-reverse: 5'-GAAGATGGTGATGGGATTTC. Results are expressed as the mean ± SD as specified in figure legends.
For proliferation assays, 2.5×104 cells/well/100 µl media were seeded in 96-well plates, allowed to adhere overnight, then washed and treated with t10c12 CLA or vehicle as specified in figure legends.
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Animals carrying UAS transgenes under the control of the TARGET system [ 36] were incubated to develop at 18°C and shifted after adult maturation (4 24 hr after eclosion) to 29°C, for 3, 10 14, or 30 days depending on the experiment and as specified in each figure and corresponding figure legend.
Each experiment was performed in duplicate or triplicates and repeated at least twice as independent experiments, as specified in each figure legend or experiment design, and the mean and standard error values were calculated.
Following in silico immunization, agents respond to features in their local environment based on low‐level rules informed by the relevant literature as specified in SUPPLEMENTARY FIGURE 1 of Read et al. 40 An example is provided in Figure 2 to illustrate this.
Figures 12(a) through 12(h) present the results of image formation and enhancement applying different DEDR-related estimators in the simulated uncertain operational scenario as specified in the figure captions.
The images of Figures 11(a) through 11(h) present the results of image formation and enhancement applying different DEDR-related estimators without model uncertainties as specified in the figure captions.
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