were determined from quadruplicate soft agar plates in single experiments or after compiling data from multiple experiments as specified.
As above specified, after 3-D culture, all the specimens were immersed in a fixative solution of 10% buffered formalin at pH 7.2 with 0.1 M sodium phosphate, for 4 h at room temperature and left overnight at 48°C.
To measure clonogenicity, U2OS cells were transfected with control or Praf2 targeting siRNAs as specified above and after 24 hours plated in 10 cm2 dishes in triplicate at 3000 cells/dish density.
Brains were harvested at the times specified after TAM and processed as described [7], [11].
The units were cleaned with 1 N NaOH as specified by the manufacturer after each run.
The same protocol was followed, as specified previously, except that, after laparotomy, bowel was evacuated into a moist cotton receptacle.
As such, an SRT condition was opposed in which motor programming is specified in advance of the go-signal with a CRT condition in which motor programming is specified after the go-signal.
Nuclei were isolated for GRO-seq at timepoints after treatment as specified.
The other eight operated patients accepted to continue IM after surgery as specified by the study protocol.
Blood was collected from the tail vein at various time-points after immunization, as specified in the figure legends, and sera were analyzed by ELISA for the presence of anti-BSA antibodies.
