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From four independent exepriments, 4 metabolites were indentified as significantly changes between samples incubated with S-Carevdilol and control, while the changes in R-Carevdilol-treated cells is less significant (Figure 4).
Data were expressed as mean±S.D., and all the P-values were given for two-sided tests and P≤0.05 was considered as significantly changes.
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Relative to CK, the proteins with fold changes of >1.5 (or <0.67) and P values < 0.05 in any sample or in any line were classified as significantly changed proteins.
A total of 42 genes were identified in IVD cells as significantly changed following culture under hyper-osmotic conditions.
In this analysis a gene was scored as significantly changed in one group in comparison to the other if it was changed in the same direction in at least 8 out of 9 comparisons performed and the fold change was above 1.5-fold.
Due to the importance ascribed to atrogin-1 and MuRF-1 in muscle atrophy, the mRNA levels of these genes were targetted using qRT-PCR in spite of them not being identified as significantly changed by the micro-array bioinformatics approach.
An FDR of <5% was designated as significantly changed.
Modification sites with p-values ≤ 0.05 were considered as significantly changed.
On the basis of the above analysis, 14 variables representing individual metabolites as significantly changed metabolites were listed out.
In total 765 genes were selected as significantly changed, exhibiting different expression kinetics in comparison with control groups.
Common genes identified as significantly changed (p < 0.001) in a paired analysis and in a non-paired block analysis.
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