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Relative to CK, the proteins with fold changes of >1.5 (or <0.67) and P values < 0.05 in any sample or in any line were classified as significantly changed proteins.
A total of 42 genes were identified in IVD cells as significantly changed following culture under hyper-osmotic conditions.
Due to the importance ascribed to atrogin-1 and MuRF-1 in muscle atrophy, the mRNA levels of these genes were targetted using qRT-PCR in spite of them not being identified as significantly changed by the micro-array bioinformatics approach.
In this analysis a gene was scored as significantly changed in one group in comparison to the other if it was changed in the same direction in at least 8 out of 9 comparisons performed and the fold change was above 1.5-fold.
An FDR of <5% was designated as significantly changed.
Modification sites with p-values ≤ 0.05 were considered as significantly changed.
Similar(29)
Further using the Cufflinks tool Cuffdiff, we identified 27 differential splicing genes which their AS patterns were significantly changed during early fruit development.
From four independent exepriments, 4 metabolites were indentified as significantly changes between samples incubated with S-Carevdilol and control, while the changes in R-Carevdilol-treated cells is less significant (Figure 4).
Data were expressed as mean±S.D., and all the P-values were given for two-sided tests and P≤0.05 was considered as significantly changes.
Mr. Annan said he would not object to the continuation of the council, which emerged from an American-led process over the summer, as long as it was significantly changed.
Chemical difference was found between traditional and dispensing granule decoctions, and albiflorin, paeoniflorin, gallic acid, amygdalin, and hydroxysafflor yellow A were identified as the significantly changed components during decocting Tao-Hong-Si-Wu decoction.
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