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Synthesized modified mRNAs, including VASA mRNA, were produced and validated prior to use as shown (Supplementary Material, Fig. S1A and B); protein expression and localization was confirmed by immunocytochemistry (Fig. 1).
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Although LOC_Os08g13440 has ∼80% AA identity with the OXLPs in the tandem array, it is oriented in the opposite direction (as shown in Supplementary Table 2).
Replicates clustered closely together as shown in Supplementary Data (Supplementary Figures S2, S3, S4).
However, there were also some differences as shown in supplementary figure S1, Supplementary Material online.
As shown in Supplementary Figure 1B and Supplementary Table 5, there were 78 upregulated GO terms and 82 downregulated terms.
As shown in Supplementary Fig. 1A, CDKL5 localized in both the cytoplasm and the nucleus (Supplementary Fig. 1A, panels).
It was also expressed at tillering and booting stages (as shown in Supplementary Fig. 6).
Certain motifs or combinations of motifs were unique to subclasses of monocupins (Fig 2; as shown in Supplementary Table 3).
The only monocupins with the same signal peptide sequence were OsOXO3 and OsOXO4 (as shown in Supplementary Fig. 1).
The presence of untranslated regions (UTRs) varied among the monocupin genes (as shown in Supplementary Table 5).
As predicted, four Hc helices interacted with each other mainly through hydrophobic residues, such as F294, I295, L298, and T301, as shown in Supplementary Fig. S2C.
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CEO of Professional Science Editing for Scientists @ prosciediting.com