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The combinations of restriction enzymes used here were Sau3A, TaqI, DdeI, EcoRI, EcoRII, and ApoI as first sites in combination with the restriction enzyme MseI as second site, and vice-versa.
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Four of the patients with lung as the first site of recurrence experienced other locations as second sites; two patients had port-site metastases to the costae after thoracoscopic pulmonary resection, one patient was later diagnosed with a new primary colon cancer, and one patient had ovarian metastasis.
Relatively high concentrations of the residues in the liver implicates it as first site of biochemical action for the drugs' metabolism.
Among them, 2 (3.8%) patients had CNS disease reported as first site of relapse.
In total, 60/222 (27%) patients had BM as first site of recurrence.
Therefore, patients with BM as first site of metastatic disease were excluded from analysis of BMFS.
The log-rank test was used for between-arm comparisons of time to CNS metastases as first site of disease progression and overall survival in patients with CNS metastases as first site of disease progression.
Of 60 patients with BM as first site of recurrence, 22 (37%) developed further systemic metastases during their course of disease.
Forty-four patients (20.7%) had BM as first site of metastatic disease and therefore were excluded from the analysis of BMFS.
Brain as first site of metastasis was defined as the presence of brain metastases in the absence of other synchronous metastases.
Overall survival, defined as time from randomization to death from any cause, was analyzed in patients with CNS metastases as first site of disease progression.
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