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When my boyfriend and me knew that i was pregnant and we relased that we were going to be parents we were as white as a sheet as first reaction, after the shock we were so happy with the good news...now, every day with our little son is a real white-knuckle ride!!! 1.
One microliter of the dilution was used as template for a second reaction using identical conditions but with primers P16 and P45.
The resulting amplification product of 603 bp was used as template in a second reaction in the presence of Leishmania-specific (R223 and R333) primers [ 14, 15].
Oxidation as the first step and a competition between an intramolecular and intermolecular Michael addition as the second reactions were studied at various pH values.
Remaining parameters were as described above for the second reaction.
20 μl of the first reaction was then used as a template for a second reaction carried out on a 1 ml scale.
An entry point is defined as the first reaction after a pathway change as annotated in the input models.
As a first reaction, we think it's illogical and we are highly sceptical about it".
Unfortunately, doctors and the lay public are automatically thinking zebras as a first reaction.
"I suspect I was being a pure contrarian … Leonardo himself always believed in scepti cism as the first reaction".
The Obama administration needs to take stock and think hard after this surprise result, especially as its first reaction was full of hasty blunders.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com