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PCR and mutational analysis were conducted in duplicate for each sample, as requested by the guidelines.
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All these RSDr values were below ≤25%% as requested by the ENGL guidelines (ENGL 2008).
For all other infants, the local ophthalmologists were asked to continue BIO as requested by the German guidelines, but in fact did use BIO at their discretion.
For all the developed SYBR®Green qPCR assays, the RSDR values were below ≤35%% as requested by the ENGL guideline (ENGL 2008): between 0 and 1.01 % for the Tm values and between 0.00 and 7.34 % for the Ct values (Table S 2).
The guidelines are also meant to ensure legal consistency across EU member states, as requested by the parliament during the legislative process of the 2013 CAP reform.
We have revised the figure as requested by the reviewer.
The investigations were repeated as requested by the clinical state.
We have removed the ANCOVA as requested by the reviewers.
These were scheduled as requested by the participants.
As requested by the reviewers we have performed ChIP assays.
Parent consent was requested for each children and were performed parent meetings as requested by the schools.
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