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The designed tyrosine derivatives have solubility in the range of 2 (low soluble) to 3 (soluble) as referred in Table 9.
Finally, colony PCR reaction was performed to verify the success of the mutations by using oligonucleotide primers 425-TT-BamHI-FW/426-TT-EcoRV-RV as referred in Table 2.
All the target genes were PCR amplified from the gDNA of S. cerevisiae R-control as a template using oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV SPT15-EcoRI-FW/SPT15-SalI-RV SPT15-EcoRI-FW/SPT15-SalI-RV SPT15-EcoRI-FW/SPT15-SalI-RV SPT15-EcoRI-FW/SPT15-SalI-RV
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We evaluate the estimator performance in three scenarios which are referred in Table 2 as #1, all links have the same statistics; # 2, the links B → R are 10 dB better than the links B → U and R → U; # 3, the overall relay links are 10 dB better than the direct ones.
We set the same values of different parameters as in [15] and [21], which is referred in Table 1.
As referred in figures.
The agar dilution method (5 ) and the E-test, as referred to in Table 1, were performed to expand the range of dilutions available in the broth microdilution trays.
The program calculates this initial fraction with respect to the one synthesized de novo during the treatment (as described in Additional file 1); it is referred in the tables as "dilution" and characterized RNA synthesis de novo.
The digits correspond to a particular trap in each sample as referred to in Figure 2 and Table 1.
GT883214-GT884367, as referred to in Additional file 1: Table S1.
2. Enzymes (EC numbers) that have not been reported in bacteria are referred to in Table S2.3 as "Missing enzymes in Bacteria".
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