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The present study reports the first construction of genetic linkage maps for Nelumbo, which can serve as reference linkage maps to accelerate characterization germplasm, genetic mapping for traits of economic interest, and molecular breeding with marker-assisted selection.
The genetic maps generated in the present study can serve as reference linkage maps of Nelumbo species for efficient studies of comparative genetics, QTL analysis of traits of economic importance, and molecular breeding with MAS.
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Taken together, the above results confirm the use of the N linkage as the reference linkage for this study.
The map was used as a reference linkage map to anchor and orient sequence scaffolds for B. rapa genome assembly.
Because of the high level of the accuracy of person links, these links were used as the reference linkage in this study.
We believe that these linkage maps will serve as reference genetic linkage maps for carnation and that many of the mapped SSR markers will be useful to compare these maps with others constructed using data from different mapping populations.
The improved genetic linkage maps and SSR markers developed in this study will serve as reference genetic linkage maps for members of the genus Dianthus, including carnation, and will be useful for mapping QTLs associated with various traits, and for improving carnation breeding programs.
For species lacking a reference genome and complete reference linkage map such as wheat, Triticum aestivum L., the majority of markers typed on a given population are unordered, and current genotype imputation methods cannot be used.
Ten linkage groups, designated as A1-A10 according to the common nomenclature of the B. napus reference linkage maps [ 17], served as a reference for the BrGSP.
However, as the mean distance between two neighboring variants is longer than the length of the simulated sequence reads and the variant calling algorithms currently do not utilize reference linkage information, this limitation does not affect the results.
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