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CM were filtered, frozen, and analyzed on antibody arrays (Chemicon; Mouse cat #AA1003M-8) as recommended, with previously described modifications [15].
Mutation frequencies (Mf) were measured using the lambda Select-cII Mutation Detection System of Big Blue® mice as recommended, with modifications.
We ran YGS, as recommended, with 15 mers.
In the Framingham Osteoarthritis Study, we acquired hip radiographs as recommended with participants standing, though this is not routinely performed.
In US young adult tourists to Mexico, vaccine efficacy against ETEC was 50% (95%CI = 14 71%), although the vaccine was not administered as recommended, with the first and second doses being taken after arriving in Mexico.
cDNA was diluted to 50 μl and 2 μl of each sample used as template for UPL assays with FastStart TaqMan Probe Master ROX (Roche) as recommended, with standard cycling and data acquisition on 7500 Fast Start Applied Biosystemss) equipment.
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As previously recommended with InterVA outputs, these data were analysed by taking the likelihoods of the assigned causes for each case and attributing any residual proportion of the death as indeterminate (12).
When there was no improvement of symptoms after taking amoxicillin/clavulanic acid for a week, metronidazole was added to the regimen as recommended, together with amoxicillin for an additional week.
First strand synthesis and labeling were performed using the Genisphere 3DNA Array 50 kit and Cy3 and Cy5 dyes as recommended (Genisphere), with 15 µg of total RNA from each sample.
Doxycycline was stopped and, as recommended, treatment with ceftazidime and oral TMP/SMX was started.
Pulsed-field gel electrophoresis (PFGE) was performed as recommended (17 ) with minor modifications.
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