Sentence examples for as readout for from inspiring English sources
Using bioluminescence imaging (BLI) as readout for firefly luciferase-transduced NPC survival, the addition of both 293-bFGF and C17.2-bFGF helper cells was found to significantly improve cell survival up to 6-fold in vitro, while wild-type (WT, non-transduced) helper cells had no effect.
The nuclear localization of NF-κB p65 visualized by anti-p65 immunofluorescence microscopy was used as readout for NF-κB activation.
Phosphorylation was used as readout for either a constitutive or stimulus-induced pathway activation, thereby providing both phenotypic and functional information [29].
We therefore performed time course experiments and tested for cleaved PARP as readout for apoptosis to further substantiate our initial finding with a different assay at different time points.
To obtain tight control of DT-A gene expression, we modified the reporter plasmid to introduce two TetO2 operator sites in the TNF-α promoter and used GFP fluorescence as readout for TNF-α promoter activity (Fig. 3A).
Due to their absence at the tip of the ASP, we could not use however pnt expression as readout for FGFR activity in hrs single mutant and hrs, stam double mutant backgrounds.
They are vacuum tubes once used as readouts for old timey electronics like nuclear reactor control rooms and James Bond villian bombs.
Furthermore, using the molecular markers p-Tyr, dpERK and Yan as readouts for EGFR activity we showed that D-cbl clones contain increased RTK and MAPK activity.
In lung cancer, CD133+, ALDHhigh, and SP have been used as markers to track CSC activity, and in vitro anchorage-independent growth, sphere formation assays and subcutaneous xenograft models have been employed as readouts for the relative CSC activity [11], [12], [13].
Hence, these synaptic parameters served us as readouts for the rescue behavior of different mutant Cplx variants.
In addition, we optimized conditions for secondary assays using laser scanning cytometry and fluorescent microscopy as readouts for cellular phenotypes (data not shown).
