The percentage of ADAS-cells being able to undergo epithelial differentiation as quantified by FACS-analysis was above 80%.
This labeled cRNA will be purified and quantified as above, normalized to equal molar concentrations, and pooled as shown in Table 3 to construct accurate standard signal intensity response curves for each oligonucleotide probe feature corresponding to the external RNA controls.
Glucose was quantified as above and change in glucose concentration was calculated using the following formula: Isolated adipocytes were obtained using the method of Rodbell [ 31] as modified by Martz et al. [ 32].
When protein fluorescence is quantified as above, wild-type ESCs cultured in 2i/LIF do indeed show more uniform patterns of Klf4, Klf5, Tbx3, and Zfp42-GFPd2 expression than cells grown in serum and LIF.
The antibody positive macrophages for CCL2, CCL3, CCL9, IL-6, NOS2, IL-4, or MMP12 were quantified as above and showed 79 to 122 per 20 fields in 9 V/null lung; INFγ or Arg2 antibody positive cells numbered 220 or 190 per 20 fields.
As the control, PrPC from healthy C57BL/6 mouse brain was purified [ 18] and quantified as above.
The remained curcumin in the supernatant was quantified as above.
Proteins were quantified as above.
Radioactive hybridization signals were detected and quantified as above.
Bound antibodies were detected and quantified as above.
Following washes, bound H-GTP was eluted and quantified as above.
