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FMR1 genotypes were designated based on a normal range of 26 34 (median 30) CGGs [21] and as previously utilized in defining specific patient populations [26]: norm was defined by both alleles within range, het by one allele outside and norm/low or norm/high, depending on the abnormal count allele being above or below normal range.
When published country-specific data were not available, we used ethnographic confidence methods, as previously utilized by Halperin and Bailey [ 5, 10], to categorize country-specific male circumcision prevalence as low, intermediate, or high.
The genome-wide distributions of the GFP-tagged histone H3 were evaluated by calculating the proportion of peaks detected on each category (promoter, gene body and inter-gene in Fig. 4a) by MACS software [ 29] with the relaxed threshold and with the broad-calling option as previously utilized by Hussein et al. [ 30] to call dispersed histone modification peaks.
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Pod F- and G-actin were measured using the methods of others [ 24, 25] and as we previously utilized [ 18].
Countries such as Sri Lanka have previously utilized volunteer CHW programmes but have experienced high attrition rates making the programmes unsustainable [ 36].
Such differences were not noted in our prior analyses of tissue CoQ levels in 180-day-old Pdss2 kd/kd mice, as we had previously utilized an oxidizing pre-column electrode to oxidize hydroquinones and generate just the quinone species (Peng et al, 2008; Saiki et al, 2008).
Alginate has been previously utilized as an adjuvant in nanoparticle-mediated gene delivery.
Ig fusion proteins have been previously utilized as effective therapeutic agents in clinical practice [23].
a To authors' knowledge, not previously utilized as a disease component for evaluating NLB resistance.
To control that the expression of shRNA was induced upon dox treatment we took advantage of GFP gene, which is cloned upstream of the shRNA and it was previously utilized as a marker of shRNA expression [ 14].
Ammonium tartrate (0.5 25 mM) has been previously utilized as an additive in direct infusion mode for improved top-down dissociation of large intact proteins (∼200 kDa) by McLafferty and co-workers as well as for reduction of sodium ion adduction on protein ions in native MS by Williams and co-workers.
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