Sentence examples for as previously modified from inspiring English sources

The well-characterized EL4 lymphoma model [24] was used as previously modified [16].

As a control, a high-speed supernatant of an extract of R. eutropha H16 grown anaerobically in FGN medium mineral salts medium [ 29] as previously modified [ 30] was included.

As we previously modified the names of pollinating wasp species, we here display the names of the Taiwanese species on the basis of the recent updates (Cruaud et al. [2011]; Segar et al. [2012]).

To generate constructs able to mediate the chromosomal integration of the above luciferase cassettes, each luciferase expression cassette was excised from its pCR2.1 vector as a HindIII– HindIII fragment and cloned into a pDC2-based vector previously modified as described in detail in the Supporting Information experimental section.

For oligonucleotide compositions handled as high-dimensional data, we have previously modified the conventional SOM for genome informatics: BLSOM.

The significant expansion of activities and revenues was agreed by the board, where the public partner acted as shareholder, without previously modifying the service contract agreed to.

All EEGs were classified by severity based on the predominant waveform from grade I to IV with I° = theta-delta-rhythm, II° = delta rhythm, III° = triphasic waveforms, and IV° = burst-suppression (modified as previously reported [ 16]).

FPU activity (Adney and Baker 1996) and β-glucosidase activity (1 IU corresponding to conversion of 1 μM substrate min-1) (Berghem and Pettersson 1973) were determined as previously described and modified (Olofsson et al. 2010).

As previously described[17], a modified ELISA was used for analysis of anti-Orthopoxvirus immunoglobulin types A and G.

Primer sequences are shown in Supplementary Table 2. ChIP assay was performed as previously with a modified protocol that uses QIAquick PCR purification kit (Qiagen) to purify precipitated DNA.

RT-PCR analysis of RNA prepared from cultured skin fibroblasts was performed as previously described with modified RT reaction (details in Data S1).