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Genes encoding oxacillinases, such as those classified as OXA-23-like, OXA-24/40-like, OXA-51-like, and OXA-58-like, were detected as previously described (7 ).
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Each aCGH profile was classified as either BRCA1-like or non-BRCA1-like and as either BRCA2-like or non-BRCA2-like as previously published based on respectively the evaluation of the BRCA1-like [ 18], and the BRCA2-like CGH pattern [ 17].
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Another set of primer sequences were used as previously reported like dopa decarboxylase [ 23], NeuroD1 and Neurogenin 2 [ 24], Hes1 [ 25], Hes6 [ 26] and Ascl1 [ 27].
As previously proposed, oocyte-like cells developed in vitro could also be matured in vivo by transplantation into other organs, tissues, or ovarian biopsies [ 5, 23]; it may be possible to autotransplant the oocyte-like cells to in vivo direct their growth, maturity, and function or perhaps to "awaken" the ovaries in women with severe ovarian infertility, if sure that teratoma would not form.
All sera were tested for immunoglobulin G (IgG) antibodies at 1 50 dilution in the ELISA using rHsp83 (ELISA-rHsp83) or whole L. major-like parasite lysate (ELISA– L. major-like) as previously described.
Specificity was also evaluated with DNA (2 μL) extracted as previously described from Francisella-like tick endosymbionts of Dermacentor variabilis and Francisella-like soil bacteria (Table A1) (9, 10 ).
The PAM50 algorithm [ 15] was used to assign molecular subtypes of n = 83 breast tumors, consisting of 29 % Luminal A, 28 % Luminal B, 27 % Basal-like, 12%% HER2-enriched, and 2 % Normal-like, as previously described [ 15].
As previously discussed, the lodA-like genes detected in fungi show an unusual genetic organization since the lodB-like gene is oriented in the opposite direction to the lodA gene.
Dendritic spines were separated on morphological categories as previously described (stubby, mushroom-like, thin or filopodia) [ 45].
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