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This risk is posed by environmental RNases, material carried over from sample to sample, as well as previously generated amplicons present on laboratory equipment.
H3 NKX2-5 eGFP/w hESCs or M1 NKX2-5 eGFP/w hESCs as previously generated (Elliott et al., 2011) were maintained on mouse embryonic fibroblasts and passaged using TrypLE select (Life Technologies).
Similar(58)
MOG1 125 was generated as previously described [9].
CSRNP-deficient mice were generated essentially as previously described [33].
Aggregates were generated as previously described [4].
shMKP-1 was generated as previously described39.
The inducible gRNA vectors were generated as previously described74.
BrdU-labelled DNA was generated as previously described6,20.
EBs were generated as previously described25,26, with minor modifications as follows.
Uncropped blots are available in Supplementary Fig. 12. Cell-derived matrices were generated as previously described54.
Viruses were generated as previously described [45].
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