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The simulations used are based on the known experimental and spin Hamiltonian parameters and on a structural model as previously employed for similar systems.
Then, the primary structure of the S. fuscescens toxin was successfully elucidated by the same cDNA cloning strategy as previously employed for the toxins of some scorpaeniform fish (lionfish, devil stinger and waspfish).
The threshold cycle (Ct) value for each gene of interest was measured for each amplified sample using the MxPro QPCR software (Stratagene) and values were normalized to GAPDH expression using the 2-ΔΔCt method as previously employed (Cucchiarini et al. 2011; Frisch et al. 2015a; Venkatesan et al. 2012).
The refractive index (RI) is used in this study as previously employed by other researchers (Buckley 1997, 1999; Buckley et al. 1998) to estimate the onset of asphaltene precipitation as a function of pore volume injection and to verify that asphaltene does precipitate and in this study RI at the onset of precipitation is denoted by PRI.
We used a ROI-based parcellation as previously employed by Margulies et al. [20] to confirm our results.
Mice were then trained on a discriminated instrumental task to press a lever to obtain the US (Figure 1C, left) using the same instrumental training protocol as previously employed to test satiety-induced reinforcer devaluation in C57BL/6J mice [30].
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We present an algorithm that correctly infers the target pattern up to 50 times as often as a previously employed method and promises sufficient power and robustness for interface control.
To balance the system, the net charge on Asp272 was increased by 0.1 and that on the carbamylated Lys153 was increased by 0.2, as has been previously employed in docking to amidohydrolases.
The PIF produced by this scheme was biologically active and induced protein degradation in murine myotubes at the same concentration as that previously employed (Whitehouse and Tisdale, 2003).
An ensemble of decision boundary (DB) was subsequently obtained, by means of SVM and the procedure based on the LOOCV (described above), using the same C (n = 5, C1,…, C5) and P (n = 4, P1,…, P4) samples as those previously employed in the training of the 'tuning stage'.
The experiments here demonstrate that no processing, as we had previously employed [ 22, 25], was necessary to yield a sufficiently resolved spectra.
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