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The protein expression in HEK 293T containig BAC GFP-EBV and GFP-EBV infected LCLs were analysed by immunoblotting along with BJAB as negative control and previously created LCLs as a positive control (Figure 5).
The artist, known as Stelarc, has previously created an exoskeleton and inserted a sculpture into his stomach.
Matan Ariel, the company's director of business development, told me that parallel videos were previously created as custom campaigns for individual clients.
The IPAT was adapted from the 18-item intensive care stress scale (ICUSS), previously created as a research tool for a prospective cohort study of intensive care psychological outcomes [ 2].
The impact of HIV/AIDS on the workforce, as alluded to previously, creates both direct and indirect forms of wastage.
This month, the interior decorator James Huniford unveils a brand-new Chelsea showroom and a sophisticated furniture collection, made up of the type of pieces the designer previously created only as one-offs for his high-profile clients.
For 2014 Russian independent watchmaker Konstantin Chaykin will release a new version of a timepiece he previously created known as the Decalogue, a watch with Hebrew characters, with a unique mechanism, dedicated to Judaica.
GFP_CBM constructs produced as described above and a previously created CelEcc_CBM3a plasmid were separately digested with SgfI and PmeI in order to obtain the nucleotide sequences encoding the CBM and CelEcc fragments.
You will notice a significant overlap with the existing model, with many of the imperfections of the previously created model smoothed as part of this operation (Fig. 20).
The homology model of MCT4 was created using a sequence alignment (Clustal Omega) [ 41] to MCT1 and building the model using the MCT1 homology model previously created [ 35] as a template.
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