We did not, as previously done, adjust for number of doses, age group, sex and village; however, these covariates do not affect peak anti-HBs level, except for a borderline effect of village.
Minimal medium was prepared as previously described, adjusted to pH 7 with sterile solutions of HCl and NaOH, and 0.5% (vol./vol). 2 µM α-tocopherol acetate (vitamin E acetate) was added to the medium.
Working bacteria culture was prepared as previously described, adjusted to be equal to 0.5 McFarland standard (1 × 10 cfu/ml) and further diluted by mixing 1 part of adjusted culture with 199 parts of TS broth to meet 5 × 10 cfu/ml.
P-values were adjusted as previously described (Benjamini and Hochberg, 1995).
To account for any effect due to differential measurement error, manual blood pressure measurements were adjusted as previously described.
Temperature, pressure and relative humidity, in the space surrounding the drop, are recorded, using a portable sensor (Omega Engineering, Inc. iBTHX-W), and reflector height computed and adjusted as previously described.
Murine peritoneal macrophages cells were isolated as previously described and adjusted to 4 × 106 CFU/mL (Guan et al. 2015; Zhang et al. 2008).
Cytotoxicity was calculated as described previously [59]: Adjusted % survival = 100×(% survival of peptide-pulsed targets/mean % survival of irrelevant peptide pulsed cells), followed by the calculation of % specific lysis = 100 – adjusted % survival.
Average sarcomere length was determined by means of a spatial Fourier transform as described previously and adjusted to approximately 2.2 µm [ 6].
For each subject, time series from these regions of interest were extracted within spheres of 10-mm radii centred on the peak for the effects of interest F contrast (P < 0.05, adjusted) as previously performed (Sladky et al., 2013).
The true cause of increased mortality is less likely to be contemporary social deprivation, as previously hypothesized, as after adjusting for multiple variables associated with social status in our analysis, the observed hazard ratios did not appreciably change.
