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(iv) Image transformation: Final job is to transform the image as per transformation model discussed above.
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This protocol resulted in extremely high transformation yields (approximately 10 individual clones per transformation).
At least 4 colonies per transformation were then sequenced.
After transformation, the cells should immediately be transferred to a flask containing 10 ml LB-broth per transformation.
Interestingly, when using sonicated S. Typhimurium DNA, the transformation rates were in the range of 10 - 10 clones per transformation.
Fresh weight growth (Fig. 1A) required a square root transformation as per a Box-Cox power transform plot analysis.
Dry weight accumulation (Fig. 2B) required a log base 10 transformation as per a Box-Cox power transform plot analysis.
Dry weight accumulation (Fig. 2A) ranged from 0.04 g – 0.27 g and required a log base 10 transformation as per a Box-Cox power transform plot analysis.
A summary of the ANOVA, lack-of-fit test and three R2 statistics are presented in Table 3. Fresh weight growth data required a square root transformation as per a Box-Cox power transform plot analysis.
As a result this data was subjected to an arcsine square-root transformation as per Field (2009).
The process seemed as essential as whatever transformation it wrought.
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