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Slides were then incubated for 30 min in EdU reaction solution (prepared as per the manufacturer's recommendation) followed by two 5-min washes in PBS.
Instead of probing the buyer with questions (as per the traditional solution selling approach), sales professionals must come to the table prepared to be a proactive source of value for customers.
Net uptake rate was measured as urea depletion from the solution per unit of time.
As the current increases, more electrons are injected from plasma into the solution per unit time; as a result, more Au NPs are produced per unit time, i.e. the production rate of Au NPs increases.
"They see us as the solution.
The CAPE solution was prepared in DMSO solution as per the previously reported procedure and manufacturer's instructions [ 30].
Working solutions, as per the experimental requirements, were freshly prepared from the stock solution for each experimental run.
The controller must fulfill these objectives to produce a feasible set of solution as per the requirements of the designer.
The remaining transformed cells were diluted with storage solution as per the Cloneminer™ II manual.
Single blastomeres were fixed using Tween 20/HCl and Carnoy's solution as per the method described by Dozortsev and McGinnis [ 16].
Following incubation with biotinylated secondary antibody and avidin biotin complex, peroxidase activity was developed with diaminobenzidene (DAB) solution as per the manufacturer's instructions.
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