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For the plot in Fig. 1B, pixel values taken from the cytosolic regions of the epithelial cells were converted to average number of photons per pixel as described in the methods section.
As per the methods sections in these studies, it appears that they did not actually individually count the cells in each lobule, suggesting a more qualitative judgement of the number of lymphocytes present.
As described in the Methods section, 21 participants per condition were needed to detect a large difference between groups, tested 1-sided alphast alpha = .05 for a power of.80.
As described in the Methods section, 15 patients per group were included in the analysis (except for the HITChip analysis, in which we excluded one outlier sample in the treated group).
We have processed all of these cDNAs for microarrays, as outlined in the Methods section, into three sets of 9,216 cDNAs per array.
The resultant fluorescence signal was analyzed to produce FCS data as described in the methods section.
Fluorescence values were normalized according to row, column, and plate as described in the Methods section.
However, these two hydraulic variables affect mangrove and saltmarsh differently, as explained in the Methods section.
Remaining parameters were condition specific and defined as described in the Methods section.
Data were normalized according to row, column, and plate as described in the Methods section.
(b) SP assays were performed as described in the Methods section.
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