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Islet isolation was as per the methods of [ 15] and [ 16].
There were six exo-enzyme assays of GAS virulence factors capable of breaking down host products to aid in bacterial survival performed using cultures grown at 37 °C with 5%% CO2; each exo-enzyme assay involved the creation of a media with specific materials to test for the virulence factor as per the methods of Grinwis et al. [ 21].
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Protein concentration in the supernatant was determined as per the method of Lowry et al. ([1951]).
DPPH radical scavenging activity was determined as per the method of Blois (1958) as modified by Binsan et al. (2008).
Degree of hydrolysis (DH) of hydrolysates was also determined as per the method of Benjakul and Morrissey ([1997]).
A portion of extract was subjected to chlorophyll removal as per the method of Lanfer-Marquez et al. (2005).
ABTS radical scavenging activity was assayed as per the method of Arnao et al. (2001) with a slight modification.
Heme iron content was determined as per the method of Cheng and Ockerman ([2004]) with a slight modification.
Peroxide value (PV) was determined as per the method of Richards and Hultin (2000) with a slight modification.
ABTS radical scavenging activity was determined as per the method of Arnao et al. (2001) with a slight modification.
To measure gelatinolytic activity, the supernatant was assayed using fish gelatin as a substrate as per the method of McLaughlin and Weiss (1996).
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