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Acid detergent fibre, neutral detergent fibre (NDF) and crude fibre were determined with an Ankom 2000 fibre analyser (Ankom Technology, NY, USA) as per the methods supplied by the manufacturer.
DLS data were analyzed by using the CONTIN method supplied by the manufacturer.
Using standard methods supplied by the manufacturer, one-dimensional SDS-PAGE was performed.
The protocol was followed as per the standard procedure supplied by the manufacturer of the kit.
qPCR was performed in either a 7900HT, 7500HT or StepOne PCR System (Applied Biosystems) and analyzed by using the ΔΔ-Ct method as supplied by the manufacturer (Applied Biosystems).
qPCR was performed in either a 7900 HT, 7500 HT, or StepOne PCR System (Applied Biosystems) and analyzed by using the ▵▵-Ct method as supplied by the manufacturer (Applied Biosystems).
Total antioxidant activity (TOA) was performed as per the method described by (Amit et al. 2009).
The regeneration frequency of protoplasts was measured as per the method described by Patil et al. (2015).
Emulsion preparation and assay were carried out as per the method described by Yamada et al. (Shimizu et al. 1979).
The DPPH assay was done as per the method described by Blois [ 20].
ELISA was done as per the method described by Dutta et al. [ 11].
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