Sentence examples for as per methods from inspiring English sources

Salinity was adjusted by adding dechlorinated municipal supply water or hypersaline (60‰) sea water (Instant Ocean, USA), as per methods modified from Kumlu et al. (2000, 2001), while water temperature was adjusted by applying either heated or chilled water to water baths until target temperatures were reached.

The doxorubicin was extracted from the tissue as per methods and estimated by fluorimetry.

Plant powder was soaked in several organic solvents petroleum ether, methanol (MeOH), ethyl acetate (EtOAc), and water for 24 up to 72 h in a ratio of 1 : 20 w/v as per methods described earlier [ 15].

The breast milk sample was collected by either hand or pump in a glass container, kept cool until delivered to the laboratory where it was transferred to 30 mL Nalgene® containers and stored at -20°C until analysis as per methods described by other studies [ 19, 20].

After primary quality filtering by established methods (as per methods and [ 26]), 1958 DArT markers were scored across a diversity panel of 182 cultivated germplasm accessions, and 1010 DArT markers were scored on 80 RILs from the KxO population (Additional file 2).

All ER+ [ER by reverse transcriptase-polymerase chain reaction (RT-PCR) ≥6.5 threshold cycle] tumor specimens that were successfully examined in the Genomic Health (GHI) central reference laboratory (as per methods previously described) [ 28] from June 1 , 2004 through December 31 , 2013ere included in these analyses.

The effluent quality was examined as per the methods described in "Standard Methods" [ 35] for determining its initial characteristics which are exhibited in Table 2.

Mucin level in gastric juice was quantified as per method reported earlier [24].

The bound rubber content was determined as per method proposed by the reported literature [30].

In method-2, same additions as per method one with additional 1 ml 30% PEG was done.

Purity of the fractions, showing chitinase activity, was checked by SDS-PAGE as per method discussed in Laemmli et al. (1970).