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There are four main MABS variables: (i) number of backcrosses, (ii) population size, (iii) number and spacing among markers, and (iv) number of marker data points (MDPs) (calculated as per marker per plant).
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Below we show how to use this method to maximize our object function, and as a result find the per marker threshold, t*1… t* m.
The relative advantages or disadvantages of the diverse marker platforms available, however, depend ultimately also on many other factors such as e.g. costs per marker data point, distribution of markers, as well as potential ascertainment bias [ 50] that cannot be discussed in the framework of this study.
An earlier study with genic-SSR markers in pigeonpea reported the average number of alleles per marker as 4 and an average PIC value of 0.40 [ 20].
Among dinucleotide SSRs, GA/CT repeat motifs exhibited more informativeness (average alleles- 2.6 per locus and PIC value- average 0.50 per marker) as compared to GT/CA repeat motifs (average alleles- 2.0 per locus and PIC value- average 0.33 per marker).
However, to make such comparisons between species that give differing average numbers of bands per individual the data are presented as the average number of bands per marker, which varied from 35 for Styela plicata and B. schlosseri (i.e., the average marker was found in 35 of 50 individuals sampled) to 18 for C. savignyi.
(D ) Trans-eQTL hotspots, shown as the distribution of significant target genes per marker region (p < 0.005) across all marker regions (red) or only marker regions containing the top 50% of ASE genes (blue).
They are comparatively cheap to genotype and provide more population genetic information per marker than biallelic markers such as single nucleotide polymorphisms (SNPs; [ 4]).
One peep per marker.
References are indicated per marker per species.
7-cm skin was analysed per marker.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com