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ASM cells were seeded into 60 mm tissue culture dishes and serum deprived for 24 h as per cell counts above.
Cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) (glucose at 4.5 g/L) supplemented with either 10% fetal bovine serum or calf serum as per cell line requirement, 100 units/ml penicillin, 100 μg/ml streptomycin, and 100 μg/ml kanamycin sulfate.
There are two main aspects of noise that can interact: an "intrinsic" component, due to the stochastic nature of the biochemical processes linked to gene expression itself, and an "extrinsic" one, which is caused by fluctuations in cellular species such as per cell concentrations of polymerases [ 26, 28- 30].
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Viability was recorded as per cent viable cells.
The number of mitochondrial DNA copies per mitochondrion as well as mitochondria per cell is exquisitely calibrated on cellular energy demand.
There was a higher percentage of CD8+ T cells that expressed LAG-3 as well as elevated per cell expression when compared to age-matched healthy individuals.
Ki67+ or EdU+ beta cell ratios were calculated as per cent total insulin+ cells.
The specific productivity qP was expressed as picograms per cell per day as described in Lattenmayer et al. (2007).
Nuclear analysis was then carried out on stained sections to establish the target protein expression firstly, as 'expression per cell', and then as 'total per tissue section'.
It had a map of Dzhabrail and Ibragim Makhmudov's whereabouts, as per cell-tower locations.
Results were reported as per cent cells in each phase of the cell cycle.
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