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Clearly, as per assay design, both DxS-KRAS and KRAS-TMGB can only detect and type 7 mutations in codons 12 and 13, thus probably missing 1% of KRAS mutations in CRC patients.
In all the different antioxidant assays, same dilutions of sample and standards were used; while standard altered as per assay requirement.
Similar(58)
Proboscis extension reflex (PER) assays were as previously described (Lu et al., 2012).
The use of high-density SNP assays such as the Illumina GoldenGate or Infinium platforms harbouring as many as 64 000 loci per assay will allow typing of a whole-genome RH panel in as little as one day.
Events 176, Mon810, and NK603 had about half as many copies per assay as did the equivalent DNA quantity for TC1507, Mon 863, and 59122 because of mass fraction differences of the certified RM (~5% versus ~10%).
Parasitaemia was assessed by flow cytometry of EtBr-treated rings (1 hour post-invasion, 5 μg/ml EtBr, staining 10 minutes, no wash) and trophozoite stages (as per 1 cycle assay).
Although it is theoretically possible to go as low as a single cell per assay, one would probably never want to go that low because it would introduce noise due to the stochastic phenotypic behavior of individual cells.
Our analysis did not include the cost of the analyzer as that cost per assay is directly related to the volume of assays performed on the instrument.
As long as there are 100 cells per assay or more, this should not be a problem.
A solution of Thioflavin T (Sigma) was prepared at 400 uM in water [64] then diluted directly into the protein solution giving a final concentration of 20 µM per assay (as done previously for insulin [63]).
As data were grouped per assay method, the between-study heterogeneity in the subgroups was lower compared with the main analysis.
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