Glucose release per gram of glucan includes an anhydro correction factor, as outlined in the procedure [ 50], to account for the addition of a water molecule upon depolymerisation.
(Taichung City, Taiwan) and validated using HPLC as outlined in the Supplemental Experimental Procedures (see Additional file 1).
Confocal microscopy was performed as outlined in the Supplemental Experimental Procedures.
Full-length env genes were PCR amplified from the single genome amplicons as outlined in the Supplemental Experimental Procedures.
Virus infectivity was determined using TZM-bl, P4-CCR5, and GHOST cells as outlined in the Supplemental Experimental Procedures.
IL-2R, CD69 expression was measured by standard FACS staining as outlined in the Supplemental Experimental Procedures.
Then, as outlined in the section 'Application procedure,' oemof's characteristics are checked for each challenge, and their contribution in tackling the challenge is debated ('Evaluation' section).
As outlined in the ACC-NCDR [12], procedures were classified as elective or non-elective based on the presentation (stable angina vs. unstable angina, NSTEMI or STEMI) and risk of infarction or death.
Outside of Ontario and in Ontario prior to 2008, CRC screening broadly followed the procedures as outlined in the national guidelines [ 7- 9], albeit in an opportunistic or individualistic manner which gave rise to concerns of underutilized or inappropriate screening and lack of quality assurance [ 23].
