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In conclusion, this study indicated that sampling faecal material from Salmonella infected chicken flocks by means of 2 pairs of socks worn on the footwear and analysed as one pool, was at least as effective in detecting Salmonella as hand collection of 60 samples of fresh faecal material analysed as one pool.
The 12 probes, which were assumed not to contain repeat elements based on the initial hybridization results, were hybridized as one pool to the filters and resulted in the identification of 396 positive BAC clones.
As one pool manager says, during the busy Cinco de Mayo weekend, "I turned away $15,000 worth of business because we didn't want too many guys in here".
Two pairs of socks analysed as one pool appeared to be just as effective in detecting S. enterica as the 60 faecal samples.
These probes were hybridized to the BAC filters as one pool, and 1203 BAC clones were verified as positive for the 15 probes after a second hybridization experiment.
The 25 honeybee brains sampled from each colony were dissected and treated as one pool, then added separately to 1 mL Trizol with 50 mg of acid washed glass beads and gently mixed for 5 min.
Similar(50)
Three types of follow-up samples were compared to each other within each flock: 1) 5 pairs of socks, analysed as 5 samples, 2) 2 pairs of socks, analysed as one sample, and 3) 60 faecal samples, analysed as one pooled sample.
By combining 4 protein pools as one mixed pool, 96 protein pools were divided into 24 mixed pools (Fig. 2A, 2B).
By combining 4 pools as one mixed pool, 96 protein pools were divided into 24 mixed protein pools for use in p62 UBA pull down assays.
Using the same assay as described above, one pool of PSMs was cultured for 3 hr in DMSO and the contralateral halves were cultured in the inhibitor.
Following an initial 30 min exposure to cycloheximide in both pools to inhibit new protein synthesis, the decay of cNICD protein was monitored as follows: in one pool protein lysate was immediately prepared whilst in the other, culture for an additional 30 60 min was performed before lysate was prepared.
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