Despite major improvement in treatment of early stage localised prostate cancer, the distinction between indolent tumors and those that will become aggressive, as well as the lack of efficient therapies of advanced prostate cancer, remain major health problems.
Fungal laccases suffer from several drawbacks, such as lack of functional or efficient expression in heterologous hosts, relatively long fermentation times and comparatively low yield.
The success of conventional suicide gene therapy for cancer treatment is still limited because of lack of efficient delivery methods, as well as poor penetration into tumor tissues.
Despite some limitations of this study such as lack of fluorescence microscopy and efficient method of isolation of nucleic acids directly from leaf, this study results indicate occurrence of natural ingression of Salmonella spp. in betel leaf.
Of that 80%, three quarters is estimated to arise as a result of the lack of efficient collection schemes and proper waste management facilities in the municipalities in many countries, with the remainder entering the marine environment from careless littering and leaks from within the waste management system itself (such as urban drains).3.3
The difficulty in diagnosing abdominal TB is due to the lack of efficient and sensitive diagnostic tools as well as its variable anatomical location.
A few factors may be contributing to the progressive erosion of the Traditional diet in Lebanon, such as trade liberalization, climate change and lack of efficient national policy to promote and sustain production and consumption of foods characteristic to the country [ 39].
However, genetic analysis of these processes remains difficult in the major crop legumes due to features such as tetraploidy, large genomes and/or the lack of efficient methods for transgenesis.
This echoes findings of similar curricular innovations in other countries such as in South Korea, where Li (1998) found lack of efficient assessment methods a significant barrier to the success of CLT.
However, those NIPFs also considered several barriers in energy wood harvesting and supply such as lack of ready energy wood market and lack of efficient logistics [18].
