However, the Flag-tagged CysLT1R was also localized to both the plasma membrane and nuclear region, similar to endogenous CysLT1R staining, as it was internalized after 5 minutes of LTD4 stimulation and could be significantly blocked by the specific CysLT1R antagonist, ZM198,615 (40 µM, 15 minutes; Fig. 4C).
"When something is written down, it is internalized as an ideal of the standard of beauty and culture," she continues.
Our antibody fulfils two important prerequisites for use as targeting molecule to cancer cells; it is internalized (Online resource, Fig. 6), and, importantly, it does not stain normal human tissues except for weak staining of one of four normal breast samples.
A caregiver who is experienced as warmly solicitous is internalized as a "good internal mother" (Chodorow 1980).
As GPR177 is internalized into endosomes (Belenkaya et al., 2008) during retrograde trafficking, association of GPR177 with RAB5 and RAB9 reflected endocytosis of GPR177 (Gasnereau et al., 2011).
After terminating Ca influx, membrane area is stable for nearly 60 s and then declines over 2 min by 70% as plasmalemma is internalized via endocytosis.
These results, performed by confocal analysis, demonstrate both the specificity of transduction by CTP as well as that the peptide is internalized, and not simply binding to the cell membrane.
Second, proteins remain bound as the NP is internalized and transported through the cell.
For nursing students, as novices within the profession, it is imperative that patient safety is internalized as both a central humanistic nursing value and a fundamental patient right [ 5].
