Sentence examples for as instructed in the manual from inspiring English sources

was used as instructed in the manual.

12 µl of each sample was loaded onto a 12-sample chip and the chips were assembled into a hybridization chamber as instructed in the manual.

The prepared samples were used in each well of the Quantikine kit, as instructed in the manual.

Fragmented DNA was used for Illumina Paired-End (PE) library preparation, using the PE library preparation kit (Illumina Inc., San Diego, CA) as instructed in the manual.

As instructed in the manual, cells were seeded in 96-well plates, stimulated with the indicated chemicals and then incubated with the CCK-8 reagent.

Re-suspended samples were denatured in a 96-well plate heat block at 95°C for 20 min. Twelve microliters of each sample was loaded onto a 12-sample chip and the chips were assembled into hybridization chamber as instructed in the manual.

Detected bands were quantified using Image J v1.43 as instructed in the software manual.

The miRNAs from blood serum were extracted using a miRNeasy Serum/Plasma Kit (QIAGEN) as instructed in the kit manual.

We applied the Fluo-4 NW into confluent cells and incubated the cells at 37°C for 30 minutes to obtain optimal results, as instructed in the product manual.

After digestion, A-tailing was done as instructed in the technical manual of pGEM-T and pGEM-T Easy Vector Systems and PCR mixtures were ligated to pGEM-T Easy Vector (Promega, Wisconsis, US), and electroporated to TOP10 cells.

The GDS was used as a direct interview, and the MDS was used as instructed in the MDS manual [ 6], and included review of the chart, semi-structured interview with the resident, direct caregivers, and available family members or key informants, in order to arrive at final symptom ratings.