Sentence examples for as initial template from inspiring English sources

Negative controls were always performed with water or macronuclear DNA as initial template.

One hundred nanogram of double-stranded cDNA was used as initial template for restriction digestion with PstI and MseI (KeyGene).

For each experimental reaction, 200 ng of total RNA was added as initial template along with 125 mM MgCl2 and 100 nM forward and reverse primers.

For the RT-Real Time PCR experiments, 200 ng of RNA extracted from root tissue of plants collected over a 48-hour time course was added as initial template for each sample with Time 0 representing the time at which tissue was collected for the microarray experiment.

For the retailer, instead of building models of large scale demographics by age, industry or gender, the goal is changing to use demographics as initial templates to build individual profiles which reflect the true needs of the person or business interacting with your marketing.

LeanIn.org may serve as an initial template.

The method begins with a protein-resistant surface containing a low percentage (1 5%) of surface-bound biotin on gold as the initial template.

Each PCR contained 30 ng of the pPhaCAB plasmid as the initial template, 250 ng of each primer, 200 μM of each dNTP, and 5 U of Taq DNA polymerase in Taq DNA polymerase reaction buffer.

The Gal4-luciferase vector (pFRluc, Stratagene) was used as an initial template for construction of the reporter vector.

Real-time quantitative polymerase chain reaction experiments were performed with 1 ng of endosperm mRNA as an initial template.

DNA "genomes" were prepared by PCR with different primer sets in two steps using the plasmid pETHisKGFP as an initial template.