The phrase "as indicated then" is correct and usable in written English.
It can be used to refer back to something that has been previously mentioned or clarified in a discussion or text.
Example: "The results of the experiment were inconclusive, as indicated then, further research is needed to draw any definitive conclusions."
Alternatives: "as previously mentioned" or "as noted earlier".
Neutrophils (10cells/ml, purity >97%) were re-suspended in a modified Hanks' balanced salt solution (in mM: NaCl 140, KCl 5, K2PO410, CaCl21.4, MgCl21.2, glucose 5, and 0.1% bovine serum albumin, pH 7.4), pre-incubated with enzyme inhibitors, as indicated, then challenged with ET-1[1-32 ET-1[1-32 ET-1[1-32times at 37ºC.
"If things are as bad as indicated then Scottish Football is in a far worse state than is being acknowledged".
Conversely, once women were put on HAART as indicated, then screening once at HAART initiation would prevent one case of cancer for every 262 women screened.
Transfected COS7 cells were treated with pharmacological inhibitors as indicated, then washed in cold PBS.
Briefly, cells were treated as indicated then washed and fixed in formal saline.
Cells were treated, as indicated, then fixed in 2% formaldehyde/PBS for 20 min at RT. Cells were rinsed twice in 10%FBS/PBS/0.02%% sodium azide.
Will you seek a second term as you indicated then you would?
As I indicated then, General Motors was suffering from a lack of buyers in what is a trillion-dollar high-yield market faced with having to absorb up to $300 billion of new supply.
The membranes were probed with specific antibodies as indicated and then incubated with horseradish peroxidase-conjugated antibody against mouse and rabbit immunoglobulin (GE Healthcare), followed by detection with ECL Western blotting detection reagents (GE healthcare).
Cells was transfected with FoxO3, MST1, and/or BRAFV600E, as indicated, and then stained with DAPI and terminal deoxynucleotidyltransferase mediated dUTP nick end labeling (TUNEL) reagents.The proportion of apoptotic cells was determined asthe percentage of TUNEL-positive cells among all DAPI-stainedcells.
1×106 cells/test were stimulated as indicated and then washed with ice-cold DPBS, spun and resuspended in Buffer A (10 mM KCl, 1.5 mM MgCl2, 1 mM dithiothreitol (DTT), 1 µg/ml leupeptin, 1 µg/ml aprotinin, 1 mM PMSF and 10 mM HEPES, pH 7.9).
