Samples were collected as indicated in the results.
As indicated in the experimental procedure, the samples were taken simultaneously on different plants that had undergone various stress treatments.
The small intestine (from the stomach to ileocaecal junction) was divided either into thirds (top, mid, and lower) or into halves, as indicated in the text, and colonic samples consisted of gut tissue distal to the ileocolic junction.
For detection of cyclin D1, cyclin E, p27kip1 and p38 phosphorylated at Thr and Tyr and AKT phosphorylated at Thr cells were treated as indicated in the figure legends, lysed in SDS PAGE sample buffer and samples were further analysed by SDS PAGE and Western blotting with specific antisera to these proteins essentially as described above.
As indicated in the previous section, for each sample we can calculate the deviation in expression against the estimated expression values calculated for each specific gene-exon pair by applying a linear regression model to the data.
The sample size, as indicated in the figure legends, was chosen based on similar experiments reported previously, and was large enough to eliminate the variance between the groups before testing.
A protocol for preparing samples and interpreting their results can now be proposed; an actual asphalt concrete has been analysed, not merely a laboratory-produced sample, as indicated in the literature.
The size and relative proportion of the minerals do vary, as observed in the sample slices and as indicated in the results previously obtained through EDXRF and AAS.
Fig. 8 Left sketch of the successive heat treatments applied to three samples as indicated in the upper inset.
