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Manufacturers' procedures were followed as indicated in the instruction manuals.
cDNA were transcribed using the IScriptc DNA synthesis kit (BioRad, Hercules, USA) as indicated in the instruction manual.
Antibodies that reacted with the antigens on polyvinylidene difluoride filters were detected by the immunoperoxidase procedure using the Vectastain ABC kit (Vector Laboratories Inc., Burlingame, CA, USA) as indicated in the instruction manual.
The effect of DJ-1 expression on breast cancer cell invasion was determined using the CytoSelect 96-well cell invasion assay kit (Cell Biolabs) containing polycarbonate membrane inserts (8- μm pore size) as indicated in the instruction manual and previously described (Cabello et al, 2009).
Set your spreader to apply the lime at the appropriate rate as indicated in the instruction manual for your spreader or on the package of the lime.
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If you are rewiring the plug, use wire cutters to ensure that wires are the correct length, as indicated in the instructions, and use wire strippers to expose about 5mm of copper wire.
As indicated in the Instructions for Authors, articles suitable for inclusion in BECT should be brief; complete manuscripts, including title, authors/affiliations, abstract, text, figures, tables, and references, must not exceed 8 (eight) pages.
As an alternative hypothesis, we can speculate that the PSYANs recognized childhood trauma better because of subtle consequences such as the specific use of particular defense mechanisms, as indicated in the instructions.
Real-time PCR was performed using the Step One Plus Real-Time PCR System (Applied Biosystems, USA), as indicated in the instructions.
Please note that students who are given permission to take an Incomplete must submit the work by the end of the fifth week of classes in the succeeding semester [of enrollment] (unless the instructor establishes an earlier deadline, or their continuation in the College is in question in which case they will have an earlier deadline, as indicated in the Bulletin of Undergraduate Instruction).
Dentocult®SM Strip mutans and BANAMet microbiological testing kits were incubated as indicated in the manufacturers' instructions and read by the field staff.
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