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Primers (Additional file 3) were validated as generating a single transcript and confirmed by the presence of one double stranded DNA product of the correct size and sequence.
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A second assembly was run using Phrap (Gordon et al. 1998) taking the velvet contigs as input, generating a single circular contig of 309,299 bp which corresponded to the chromosome sequence of the flavobacterial endosymbiont.
If the individual fires are extensive enough, they can coalesce into a mass fire known as a firestorm, generating a single convective column of rising hot gases that sucks in fresh air from the periphery.
In other words, WFCCM extends the compound covariate method by allowing for more than one statistical analysis method to be considered in the covariate and reduces the dimensionality of an analytic problem by generating a single covariate calculated as a weighted sum of the class predictors identified as most important.
By splicing several combinations of exons into different transcripts, AS generates, from a single gene, multiple isoforms of a protein with potentially diverse functions.
Instead it might be practical to combine the data gathered on the population as appropriate and generate a single QTL map from the combined data.
While generally demonstrating a similar sensitivity and specificity to the CellSearch system, our assay offers several other advantages: first, our assay is objective, as an algorithm generates a single score automatically for determining CTC positivity based on tumour marker gene expression levels detected by QPCR.
Together the items are used to generate a single global score as well as 5 sub-scores representing the following domains: physical, psychosocial, medical, marital and sexual functioning.
With respect to hybridisation specificities, the Nugen technology shows a fundamental difference as it generates a single-stranded cDNA target whereas others protocols yields cRNA targets.
As proof of concept, we have generated a single DNA fragment containing the genes Trap, Dmp1, and Ibsp driving the expression of ECFP, mCherry, and Topaz FP reporter genes, respectively.
Composite index models generate a single index, such as the ecological footprint [5] or the green gross national product.
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CEO of Professional Science Editing for Scientists @ prosciediting.com