Sentence examples similar to as first strand from inspiring English sources

First strand cDNA was originated from 2 ug of RNA using the Ready-To-Go You-Prime First-Strand Beads© kit (GE Healthcare) and pdN[6] as first-strand primer.

These were used as first-strand cDNA templates synthesised by using random hexamer primer.

cDNA synthesis according to the SMART protocol is as convenient as conventional first strand synthesis.

The endogenous human retroviruses exist in multiple types, generally named after the amino acid of the tRNA that serves as primer for first strand synthesis.

For each RNA sample, total RNA was then used as template for first strand cDNA synthesis.

Three microliters of total RNA sample (0.9 μg) was used as template for first strand cDNA synthesis using the BD-Clontech CreatorTM SmartTM cDNA library kit following the manufacturer's protocol.

For each sample, 1 μg of purified RNA was used as template for first strand cDNA synthesis with random hexamer primers and SuperScript III reverse transcriptase (Invitrogen) following the standard manufacturer's protocol.

Two micrograms of RNA was used as template for first strand cDNA synthesis to make 3'RACE-ready cDNA using The SMART RACE cDNA Amplification Kit according to the manufacturer's protocol (Clonetech).

RNA samples with distinct 18S and 28S ribosomal RNA bands on denaturing agarose gels were then used as templates for synthesis of first strand cDNAs as described previously (6, 9).

The substrate specificities of the shrimp nuclease, the crab nuclease [14] and DNase I was compared using this substrate, either as ssDNA (first strand only) or as dsDNA (first and complementary strand).

The gene-specific RT primers were equally mixed used as RT primers for first strand cDNA synthesis.