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Analyses of two experimental HRTEM images are mentioned in Ref. [20] as examples where reciprocal lattice vector magnitudes as extracted with CrysTBox are compared to their theoretical reference values.
At the end of the study, 26.5% of teeth (n = 236) were recorded as extracted with known reasons.
100 mg of powder as extracted with 300 μl methanol containing 200 ng of β-apo-caroten-8-al as internal standard for each sample and 300 μlTris-HCl (50 mM, pH 7.5, 1 M NaCl).
To test for differential expression [false-discovery rate (FDR -adjusted P < 0.1, absolute log2 FDR -adjusted1], we aPplied the edgeR package in R (Robinson et al. 2010) on the raw counts as extracted with HTSeq (Anders et al. 2015) for the IWGSC high confidence genes.
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However, significant differences were seen with regard to the antiradical activity and reducing power of the extracts, as extract obtained with LTTM1 displayed consistently strong effects, whereas the extract obtained with LTTM3 had rather questionable efficiency.
Osteoclast precursors were pretreated with AS extract and stimulated with RANKL at various time points.
Osteoclast precursors were pretreated with AS extract and further stimulated with RANKL at various time points.
When WbtIG191V_P17 was grown on Glc-CDMA as described above, extracted with 0.5% phenol, and the cells removed by centrifugation, the extract was thick, frothed easily, and was slightly yellow in color.
To immunoprecipitate, cells treated as indicated were extracted with lysis buffer and incubated with recommended amount of primary antibodies by manufacturer for overnight at 4°C and then further incubated with protein A/G agarose beads (Pierce) for 2 hrs.
The pellet from one litre of an exponentially growing culture obtained as above was twice extracted with 5% TCA o/n at 4°C.
As the NA cultures were extracted with 70% acetonitrile in water (v/v), the ST34LC (original crude extract) was further extracted with 70% acetonitrile (ST34LC-AE) and analysed.
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