Finally, the water phase was applied to a QIAshredder spin column and the rest of the steps performed as explained in the RNeasy protocol.
MIC assays with 1, 2, and 3 were performed as explained in the MBC calculation protocol, according to recommendations from the CLSI for aerobic growth of bacteria.
All subjects who attend the first session of the research clinic will be asked by the principal investigator, JN, to sign a consent form and will be invited to raise any concerns about the protocol (as explained in the full information sheet).
We need this information for implementing our protocol as explained in the follow.
The route discovery and weight assignment algorithm was borrowed from an existing protocol, as explained in the following section, because it is very well suited for WSN, in which information has to converge into a node with special responsibilities (the sink) and because the relevant protocol assigns weights to the different discovered paths.
Reconstitution of holo-RNA polymerases using M. smegmatis core (α2ββ'ω) and the M. tuberculosis σA and σB were performed following the same protocol as explained in the previous study.
After tumors had grown to 80 100 mm, the animals were subjected to the different treatments using injection protocols, as explained in the Materials and Methods.
The initial steps of the analysis followed the previously described protocol (Peña-Miller et al. 2013) as explained in the supplementary material, Supplementary Material online.
In addition, the proposed protocol makes use of HM levels 2/4-QAM and 4/16-QAM in the first step, as explained in the next section.
Total RNA was isolated using Qiazol (Qiagen) according to the manufacturer's protocol, with modifications as explained in [ 9].
