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The Cox regression modeling as explained in Material and Methods revealed that anti-Ma2 titer is more predictive of patient outcome than CgA concentration.
The reproducibility of the assay is expressed in intra- and inter-assay percent coefficient of variation (CV) as explained in Material & Methods.
A coexpression network analysis was performed to obtain information about the relationships between the different miRNA (as explained in material and methods).
As explained in Material and Methods, in the case of H358 and H2170 cells, some clones (shTMP4-2 and shTMP4-3 for H358 and shTMP4-8 for H2170) with a reduction higher than 60% in TMPRSS4 levels were selected and used for further experiments.
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After the mice were implanted with U251 and 5310 cells as explained in Materials and Methods, the mice were observed for 30 days.
As explained in Materials and Methods we have used the MOS algorithm [20] to generate such an ensemble of random walks.
These were processed as explained in Materials and Methods, so that the effect of the transgenic proteins on Drosophila somatic sexual development could be studied.
This kind of sampling, as explained in Materials and Methods and Document S1, is insensitive to most of the common mode frequency modulations.
After the mice were implanted with U251, 5310 and hUCBSC as explained in Materials and Methods, the mice were observed for 21 days.
The predicted spectral distribution of aequorin (luminescence) and GFP (fluorescence) (Figure S2B) matched their experimental emission into the four imaging channels (as explained in Materials and Methods) (Table 1).
The genetic background of Peruvian Mestizo is close to that of Mexican Mestizo [22]; however, since subtle differences in the admixture of cases and controls may introduce bias we applied a methodology developed by Reich and Golstein to adjust for differences in the genetic admixture of cases and controls as explained in Materials and Methods [23].
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