Exact(5)
On the other hand, fibroin based materials, irrespective of their physico-chemical characteristics appeared to be unable to induce the activation of peripheral blood T cells from healthy donors, as evaluated by the expression of activation markers and IFN-γ gene.
The efficiency of transfection as evaluated by the expression of EGFP in cells transfected with pmaxGFP vector was more than 50%.
Moreover, the tested ligands differed in their ability to induce cellular activation as evaluated by the expression of the cluster of differentiation markers (CD) 25, CD38, CD39, CD69, CD154, and human leukocyte antigen D related (HLA -DR as well as of a cell proliferation marker, Ki67, and of CCR5.
The results presented in this study show that LC generated in the presence of Activin A are fully competent to undergo a maturation process, as evaluated by the expression of CCR7 and the downregulation of CCR6, the expression of CD80 and CD83, the ability to induce T cell proliferation and to secrete high levels of chemokines (i.e. CCL20 and CCL22) and cytokines (TNF-α, IL-12p70).
For the first time, we have shown that Tr1 levels, as evaluated by the expression of the CD49b surface marker in serum, PBMC, or liver before the introduction of antiviral therapy, were significantly lower in patients who would develop a sustained virological response (SVR).
Similar(55)
In most cases, the correlation between gene expression levels quantified by the array and by QPCR is strong, as evaluated by the correlation between two expression profiles (Pearson's correlation coefficient) and the linear correlation R of each probe/gene pair.
However when total germ cell population from 4dpp testes, which includes Kit positive cell contaminant, were cultured in RCCS, they progressed to early meiotic stages, as evaluated by induction of Scp3 expression and its organization in preleptotene-like figures, which were never observed at unit gravity.
PC3 cells transfected with this plasmid were separated from untransfected cells by the MACSelect Kk System (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and cultured for 3 4 weeks with 0.4 μg/mL hygromycin B. Before transfection of siRNA or the expression plasmid, over 80% of the cells contained the BCPR-luciferase reporter plasmid as evaluated by EmGFP expression.
In agreement with their in vivo findings, we did not detect major differences in astrogliosis, as evaluated by GFAP expression between control and PSA-NCAM expressing cells.
In fact, when Kit-negative spermatogonia (obtained from 4dpp testes, after removal of Kit positive cells) were cultured for 24 h in RCCS, they underwent differentiation, as evaluated by increased expression of genes important for commitment of mitotic germ cells to the meiotic programm (Kit and Stra8), even though they failed to organize Scp3 onto chromosomes.
This finding is in accordance with the results of a recent study which demonstrates that mESC infection with other T3SS expressing bacteria, such as Shigella flexneri, does not alter pluripotency, as evaluated by Oct4 expression levels [23].
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com