EBs were purified from Urografin gradients as previously described [31] and their infectivity was evaluated by serial titration and staining of inclusions using an in-house monoclonal antibody specific to chlamydial LPS (Mab29) [32].
To identify the specific neural substrates of structural changes, animals evaluated by serial MRI underwent post-mortem immunohistochemical evaluation of the integrity of the nigrostriatal system.
Response was evaluated by serial hCG monitoring.
Spacer sequence stability was evaluated by serial subculture.
The sensitivity of PCR amplification of each promoter was evaluated by serial dilution of total DNA collected after sonication (input fraction).
Optimal antigen- and serum concentrations had been evaluated by serial dilutions prior to the study.
To evaluate the efficacy of topiramate, the neurologic outcome of enrolled newborns will be evaluated by serial neurologic and neuroradiologic examinations.
Cytotoxic activities were evaluated by serial-dilution method in 96-well flat-bottom microtiter plates.
(c) Cardiomyocytes ultrastructure changes as evaluated by transmission electron microscopy.
To evaluate by serial ultrasound scans how long a Ringer's lactate instillation remains in the peritoneal cavity after laparoscopy.
