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The mice were challenged with M1 GAS on day 0. ELISAs were performed for antibody determination as essentially described [2].
Luciferase refolding was assayed as essentially described by Parsell et al. [ 34].
Routine PCR genotyping of Bapx1 lines was performed as essentially described in [ 17].
The plasmid shuffle technique was employed as essentially described by Loovers et al. [ 32] with minor modifications.
An orthotopic xenograft model of liver tumors with Matrigel was used to explore tumor growth inhibition in situ, as essentially described in our studies.
Immediately after harvesting culture samples for RNA purification or at least 30 min later, polysomes were isolated as essentially described by Maki [ 49].
Similar(49)
These were performed as essentially as described by Park et al. [ 9].
Reverse transcription was carried out as essentially as described for chemical mapping experiments.
Protein purification was performed essentially as described previously4, 20.
Autoradiography was performed essentially as described previously [20].
SDC were generated essentially as described [56].
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