Sixteen genes, chosen to represent a wide range of gene expression, were selected for verification of transcript abundance using quantitative real time PCR (qRT-PCR), as documented in our previous analysis of this data for uterus-specific over-expression [ 21].
It is however difficult to provide an explanation for the differences in regulation pattern of these two PR-protein transcripts, but as documented in other studies the increases in PR5 could be transient.
In our study, we identified a total of 22523 novel transcripts (~25% of total transcripts) in mouse lens as documented in Table S2 along with their novelty score and expression levels.
In most cases this technique is sufficient, as the authors documented in this article.
Similar to our previous findings in epithelial cells, reprogrammed HMFs exhibited an activation of the activin A pathway, as documented by increases in activin A and COX-2 transcript levels in HMFs co-cultured with TRF2-vHMEC.
They undergo AS, as documented by genome-wide analysis of transcript variants [ 27].
Neither MCAM/MUC18 mRNAs were detected when mRNA was isolated from breast carcinoma cell line (MB-231 and MCF-7), as documented [ 15, 17], nor MCAM/MUC18 transcripts were evidenced in the blood of our healthy donors [ 49].
Their exchange is documented in audio recordings and transcripts, and the face-off spurred a bitter rivalry that continues to this day.
TNF-α increased Nox4 protein and transcripts in HIMECs as documented by blot and real-time PCR (P<0.01 vs. control; Figure 6e,f), and these effects were partially counteracted by PLC pretreatment (P<0.05).
(The transatlantic connections are another fascinating story, as seen in this transcript of a 1978 discussion with Leacock and Rouch).
