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Twenty of the 21 targeted genes were complete as determined by sequence alignment indicating full coverage of FL cDNA sequences from spruces and pines (P. glauca, P. sitchensis, P. taeda and Pinus sylvestris) [ 25- 28] (Additional file 1: Table S7).
The putative domains of irrE from D. radiodurans were determined by sequence alignment with irrE from D. deserti as described (see Figure 9).
The percentage of nucleotide (nt) and amino acid (aa) identity between mecD and other mec genes was determined by sequence alignment with Clustral OMEGA [ http://www.ebi.ac.uk/Tools/msa/clustalo/].ac.uk/Tools/msa/clustalo/
The transmembrane regions are determined by TMHMM [17] and the glutamate binding regions are determined by sequence alignment.
Primers for SYBR green assays of stefin genes were carefully designed to include nucleotides specific to the desired gene as determined by multiple sequence alignment (Text S1).
Therefore, the amino acid sequence diversity encoded by aquaporin transcripts was determined by multiple sequence alignment of orthologs using ClustalW.
The nucleotide sequences present at exon-intron boundaries, as determined by the alignment of the gene nucleotide sequences determined by the TBLASTN search with their cognate contigs, were found to be highly conserved.
(iv) Further sequences are kept if they cover the start and end of the gene as determined by the alignment positions gene-start and gene-end until at least fs-cover-gene such sequences have been found.
%ID (%SIM) refers to percentage identity and similarity with the human β1 protein sequence as determined by alignment.
Genome coordinates of RefSeq transcripts as determined by BLAT alignment were obtained from UCSC on 12/29/05.
Evolutionary relationship was determined by sequences of GPATs obtained from BLAST and alignment using Clustalx 1.83 software.
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