As detailed in the "Methods" section, above, the two backscatter detectors (Gatan BSD and FEI CBS) were configured differently.
This derives from an examination of the information provided in the FCEC report to the Commission [[11]], as detailed in the 'Methods' section.
(5) and (6), as detailed in the methods, and resulting K d were calculated as the ratio of k off/k on.
The tag-SNPs were selected using linkage disequilibrium as detailed in the methods section.
Liver wedges were prepared as shown in Figure 1 and processed as detailed in the Methods section.
As detailed in the Methods section, this model relies on the estimation, through a logistic regression, of both a "locus effect" and a "population effect" on genetic differentiation.
Additional referent subjects were selected from the Illumina iControlDB database using genome-wide genetic matching as detailed in the methods (n = 1635).
Skov-3 tumor proliferation was measured based on fold change compared to normal Skov-3 cell proliferation in low serum media as detailed in the methods section.
(In this regard, environmental conditions were relatively benign in our study, with temperature consistently <25°C, as detailed in the Methods section).
It should also be noted that even though all cloning was directional, cloning success was much improved by complete digestion (typically overnight), de-phosphorylation, and purification of the recipient vectors prior to all ligations (as detailed in the methods).
As detailed in the Methods Section, using Metamorph (http://www.moleculardevices.com/pages/software/metamorph.html), we measured the size of the unhealed tissue in each animal more precisely for this experiment than for Experiment 1.
